The ruling by U.S. District Judge Richard Bennett was a victory for rivals Perdue Farms and Sanderson Farms Inc., who are suing to stop the advertisements.
Tyson operates a poultry processing plant in Shelbyville.
Salisbury-based Perdue and Laurel, Mississippi-based Sanderson argued the advertisements are misleading because none of the companies uses those types of drugs and shoppers could be led to think other companies are using the drugs.
Bennett said he was convinced "that the consumer public is being misled" by the "Raised Without Antibiotics" advertising.
All three companies use feed containing ionophores, which are largely considered to be antibiotics. However, the substances are not used in humans and thus are not believed to raise human health concerns.
Last spring, the U.S. Department of Agriculture said Tyson, based in Springdale, Ark., could label its foods as "raised without antibiotics," but the USDA later reversed that decision after Tyson had spent money on advertising and packaging. Tyson was eventually allowed to say its products are "raised without antibiotics that impact antibiotic resistance in humans."
Sanderson has argued it lost a $4 million account to Tyson because of the advertising campaign, and Perdue claims it has lost about $10 million in revenue since last year.
Bennett ruled the qualifying language was not understood by a substantial portion of the consumer public and the advertising "may even reinforce consumer misconception."
Tyson issued a statement saying the preliminary injunction does not affect the USDA-approved product label used on Tyson's fresh chicken products. However, the decision does affect point-of-sale materials in stores and the company will seek a stay of the order.
Dave Hogberg, senior vice president of Consumer Products for Tyson Foods, said in the statement that "we firmly believe we have acted responsibly in the way we have labeled and marketed our products."
Attorney Randall Miller, lead counsel for Perdue and Sanderson, said his clients felt the modified language was "more insidious" because it connected the dots to antibiotic resistance, an important consumer safety concern.
Miller has said his clients could make similar claims but feel they are deceptive and have refused to do so. Tyson had said the two rivals were trying to use the courts "to circumvent the regulatory judgment of USDA" and asked Bennett to dismiss the case, a request the judge denied last week.
Tyson's shares closed Tuesday down 67 cents, or 3.5 percent, at $18.77. Sanderson Farms also lost $1.23, or 2.9 percent, to $40.70.

Well you have to give chickens antibiotics because they can die from anything. It's the stuff they put on the meat after it's processed is what bothers me.
The problem with the advertising was the timing of them. Tyson knew that their competitors were engaging in negotiations and used this as a tactic. Think about it.
tgreader, I agree with you. If the other company's don't want people thinking they use antibiotics in their food then they should spend their own money advertising it. I don't think it's right to stop another company from advertising the quality of their products, because someone may think less of another company. It ain't like the advertisement said the other company's use the antibiotics.
Maybe they could advertise that their animals are organically and humanely raised,they use Fair Trade practices,they meet the dietary rules of kashrul,I-tal and halal (based on the Bible) and that hormones,antibiotics,etc. aren't required for them to produce healthy livestock.
That would impress consumers more than if they advertized "No cyclamates. No antibiotics. No hexachlorophine. No red dye #2. Now with Downy freshness!"
I don't see what is preventing the other companies from using the same wording for their advertising. Basically the court is telling Tyson you can say you aren't doing such and such because it may imply everyone else is. That's ridiculous!
Surely Tyson Food wouldn't lie (sarcasm)