Shelley Mook's Pontiac Grand Prix was recovered off Manchester Highway, said Tennessee Bureau of Investigation spokesperson Kristin Helm.
A creek near the car was searched, investigators said Thursday night, adding that rumors of Mook's body being found are untrue.
Mook, 24, who moved to Murfreesboro last week, was last seen about 3 p.m. Monday, Detective Sgt. Brian Crews said Wednesday.
"She had been living in Shelbyville and just moved to Murfreesboro over the weekend," Crews said.
"She has an ex-husband that lives in Bedford County. As far as we can tell, he is the last person to see her when she went to his house to drop off their daughter," Crews said. "She was supposed to meet a maintenance man at her new apartment on Monday afternoon at 4:30 but, according to the landlord, she never showed up."
A woman who said she was Mook's closest friend told the Times-Gazette this morning she is confident she'll be found.
"She's a strong woman," Brittany Brooks said. "She's going to come through."
Brooks asked for prayers for her friend: "We need everybody to pray for her."
Mook teaches eighth grade reading "and absolutely loves her job," according to Brooks.
"Shelley Mook is an excellent member of the Harris Middle School faculty and well-liked by students and staff," principal Bill Pietkiewicz said this morning. "She is in her second year of teaching and is helping to sponsor our student council. Our thoughts and prayers are focused on her safe return."
Crews said Mook had taken time off from work to move, Crews said. Co-workers became concerned after she didn't return or schedule a substitute.
Anyone with information should call Shelbyville Police Department's detective division at 684-5811.